3QF6
Neutron structure of type-III Antifreeze Protein allows the reconstruction of AFP-ice interface
Experimental procedure
| Experimental method | LAUE |
| Source type | NUCLEAR REACTOR |
| Source details | ILL BEAMLINE LADI III |
| Synchrotron site | ILL |
| Beamline | LADI III |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-05-23 |
| Detector | LADI III |
| Wavelength(s) | 3.18-4.22 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.729, 39.123, 46.537 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.950 - 1.850 |
| R-factor | 0.1674 |
| Rwork | 0.162 |
| R-free | 0.21630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.353 |
| Data reduction software | LAUEGEN |
| Data scaling software | LSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.950 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.140 | 0.195 |
| Number of reflections | 4803 | |
| <I/σ(I)> | 17.5 | 5.5 |
| Completeness [%] | 89.3 | 71.4 |
| Redundancy | 14.8 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 285 | 2.2M ammonium sulfate, 9% d8-glycerol, 50mM sodium acetate, pD 5.2, pH 4.8, VAPOR DIFFUSION, SITTING DROP, temperature 285K |
