3QF2
Crystal structure of NALP3 PYD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2010-09-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.032, 60.040, 51.529 |
| Unit cell angles | 90.00, 107.62, 90.00 |
Refinement procedure
| Resolution | 25.610 - 1.700 |
| R-factor | 0.18592 |
| Rwork | 0.183 |
| R-free | 0.23500 |
| Structure solution method | SAD |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.142 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.745 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Total number of observations | 100286 | |
| Number of reflections | 26472 | |
| Completeness [%] | 75.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 0.2M ammonium citrate, 0.1M sodium acetate pH4.6, 30% PEG MME 2000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
