3QEU
The crystal structure of TCR DMF5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-27 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 184.155, 86.496, 66.475 |
| Unit cell angles | 90.00, 103.97, 90.00 |
Refinement procedure
| Resolution | 29.790 - 2.090 |
| R-factor | 0.209 |
| Rwork | 0.206 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.668 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.140 |
| High resolution limit [Å] | 2.090 | 5.690 | 2.090 |
| Rmerge | 0.054 | 0.036 | 0.292 |
| Number of reflections | 59138 | ||
| <I/σ(I)> | 20.7 | ||
| Completeness [%] | 99.7 | 96 | 99.7 |
| Redundancy | 3.7 | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | PEG4000 30%, TRIS 0.1M, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
