3QEK

Crystal structure of amino terminal domain of the NMDA receptor subunit GluN1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9788
Spacegroup name	P 21 21 21
Unit cell lengths	47.323, 92.813, 209.978
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.948 - 2.001
R-factor	0.1938
Rwork	0.192
R-free	0.22640
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.064
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.070
High resolution limit [Å]	2.000	4.310	2.000
Rmerge	0.396	0.039	0.396
Number of reflections	120092
<I/σ(I)>	16.6	37.6	4.3
Completeness [%]	99.9	99.4	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	290	20% PEG3350, 150mM KNO3, 50mM HEPES pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K