3QEE
The structure and function of an arabinan-specific alpha-1,2-arabinofuranosidase identified from screening the activities of bacterial GH43 glycoside hydrolases
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-16 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.290, 139.270, 50.460 |
| Unit cell angles | 90.00, 115.95, 90.00 |
Refinement procedure
| Resolution | 69.635 - 1.640 |
| R-factor | 0.15439 |
| Rwork | 0.153 |
| R-free | 0.18402 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qed |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.226 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.635 | 1.730 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.097 | 0.403 |
| Number of reflections | 71449 | |
| <I/σ(I)> | 11.5 | 4.1 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 13% PEG3350, 150 mM sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
