3QE7
Crystal Structure of Uracil Transporter--UraA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.99294 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 96.760, 96.760, 251.950 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.920 - 2.781 |
| R-factor | 0.2506 |
| Rwork | 0.248 |
| R-free | 0.30350 |
| Structure solution method | SAD |
| RMSD bond length | 0.033 |
| RMSD bond angle | 0.956 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.3_473)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.920 | 2.880 |
| High resolution limit [Å] | 2.781 | 2.780 |
| Rmerge | 0.078 | 0.783 |
| Number of reflections | 18225 | |
| <I/σ(I)> | 17.8 | 1.9 |
| Completeness [%] | 98.7 | 99.1 |
| Redundancy | 4.3 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 25% PEG 400, 100mM MES-NaOH, 300mM Li2SO4, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
