3QE6
Mouse PACSIN 3 F-BAR domain structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-09 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.849, 54.653, 193.655 |
| Unit cell angles | 90.00, 96.92, 90.00 |
Refinement procedure
| Resolution | 28.010 - 2.600 |
| R-factor | 0.22983 |
| Rwork | 0.227 |
| R-free | 0.27415 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q84 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.041 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.770 |
| High resolution limit [Å] | 2.500 | 2.770 | 2.600 |
| Number of reflections | 28175 | ||
| Completeness [%] | 97.0 | 99 | 92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 3350, MgCl2, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
