3QE1
Crystal Structure of PDZ domain of sorting nexin 27 (SNX27) fused to the C-terminal residues (ESESKV) of GIRK3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-08-26 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | H 3 |
| Unit cell lengths | 75.789, 75.789, 40.939 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.900 - 1.680 |
| R-factor | 0.1609 |
| Rwork | 0.158 |
| R-free | 0.22276 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ocs |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.976 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.039 | 0.174 |
| Number of reflections | 8921 | |
| <I/σ(I)> | 39.53 | 6.68 |
| Completeness [%] | 86.3 | 45.2 |
| Redundancy | 4.8 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.2 M calcium chloride, 0.1 M HEPES, 28% v/v PEG400, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
