3QDK
Structural insight on mechanism and diverse substrate selection strategy of ribulokinase
Replaces: 3JVPExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-27 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9791 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.683, 88.840, 230.525 |
Unit cell angles | 90.00, 92.75, 90.00 |
Refinement procedure
Resolution | 48.310 - 2.310 |
R-factor | 0.224 |
Rwork | 0.224 |
R-free | 0.27400 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHELXD |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.350 |
High resolution limit [Å] | 2.310 | 2.310 |
Rmerge | 0.065 | 0.165 |
Number of reflections | 99100 | |
<I/σ(I)> | 13.4 | 4 |
Completeness [%] | 98.1 | 87.8 |
Redundancy | 3.6 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 30% PEG4K, 0.2M Sodium acetate, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 30% PEG4K, 0.2M Sodium acetate, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |