3QDH

Crystal structure of Actinomyces fimbrial adhesin FimA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Detector technology	IMAGE PLATE
Collection date	2009-06-11
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	47.528, 39.799, 77.139
Unit cell angles	90.00, 98.34, 90.00

Refinement procedure

Resolution	40.000 - 1.900
R-factor	0.21132
Rwork	0.209
R-free	0.24902
Structure solution method	MIRAS
RMSD bond length	0.008
RMSD bond angle	1.110
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	autoSHARP
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.063	0.203
Number of reflections	22677
<I/σ(I)>		11.8
Completeness [%]	99.3	99.5
Redundancy	3.5	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	17% PEG 2000 MME, 0.1M imidazole, 0.2M zinc acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	8	295	17% PEG 2000 MME, 0.1M imidazole, 0.2M zinc acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K