3QDA
Crystal structure of W95L beta-2 microglobulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2010-09-11 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.410, 28.980, 54.450 |
| Unit cell angles | 90.00, 121.77, 90.00 |
Refinement procedure
| Resolution | 26.650 - 1.570 |
| R-factor | 0.14941 |
| Rwork | 0.147 |
| R-free | 0.19781 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lds |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.505 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.654 | 1.650 |
| High resolution limit [Å] | 1.569 | 1.570 |
| Rmerge | 0.056 | 0.251 |
| Number of reflections | 14630 | |
| <I/σ(I)> | 17 | 5.4 |
| Completeness [%] | 99.8 | 98.3 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 19-20% PEG4000, 20% glycerol, 0.2 M ammonium acetate, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
