3QD2

Crystal structure of mouse PERK kinase domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-03
Detector	MAR CCD 165 mm
Wavelength(s)	0.998
Spacegroup name	P 41 21 2
Unit cell lengths	97.891, 97.891, 116.706
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.630 - 2.810
R-factor	0.2729
Rwork	0.270
R-free	0.33230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.151
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	44.630
High resolution limit [Å]	2.800
Number of reflections	13283
Completeness [%]	97.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	1.1M sodium citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K