3QCW
Structure of neurexin 1 alpha (domains LNS1-LNS6), no splice inserts
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.953, 114.543, 159.581 |
| Unit cell angles | 90.61, 90.87, 92.18 |
Refinement procedure
| Resolution | 30.000 - 2.650 |
| R-factor | 0.2093 |
| Rwork | 0.208 |
| R-free | 0.22960 |
| Structure solution method | SAD, MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.730 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.700 |
| High resolution limit [Å] | 2.650 | 7.190 | 2.650 |
| Rmerge | 0.079 | 0.036 | 0.526 |
| Number of reflections | 124701 | ||
| <I/σ(I)> | 14.5 | ||
| Completeness [%] | 98.5 | 98.6 | 98.2 |
| Redundancy | 3.4 | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 5-10% PEG8000 or PEG10000, 2.5 mM calcium chloride, 100 mM bicine, pH 8.0-9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
