3QC6
GspB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.03034 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.338, 86.743, 117.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.810 - 1.900 |
| R-factor | 0.2174 |
| Rwork | 0.215 |
| R-free | 0.26120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.362 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.068 | |
| Number of reflections | 26193 | |
| Completeness [%] | 93.8 | 92.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291.15 | 10 mg/mL protein buffered in 20mM HEPES pH 7.5 equilibrated against a reservoir solution containing 25% PEG3350, 0.1M HEPES pH 7.5, 0.15M NH4CH3COO, and 10mM Spermidine, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K |
