3QC5
GspB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Detector technology | CCD |
| Collection date | 2009-06-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.681, 96.753, 100.192 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.700 - 1.400 |
| R-factor | 0.144 |
| Rwork | 0.142 |
| R-free | 0.17200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.378 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 60816 | |
| Completeness [%] | 92.7 | 54.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 296.15 | 6 mg/mL GspB buffered in 20 mM Tris, 33% Jeffamine ED-2001, 0.1 M HEPES, 0.15 M KCl, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 296.15K |
