3QC3

Crystal structure of a D-ribulose-5-phosphate-3-epimerase (NP_954699) from HOMO SAPIENS at 2.20 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-03
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97944,0.97894
Spacegroup name	P 41
Unit cell lengths	47.665, 47.665, 220.774
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.127 - 2.200
R-factor	0.1667
Rwork	0.164
R-free	0.21120
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.614
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.127		2.280
High resolution limit [Å]	2.200	4.720	2.200
Rmerge	0.095	0.030	0.651
Number of reflections	24823	4903	4849
<I/σ(I)>	8.35	23	1.6
Completeness [%]	97.6	96.9	96.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	0.16M magnesium acetate, 10.0% polyethylene glycol 8000, 20.0% Glycerol, 0.1M sodium cacodylate pH 6.0, NANODROP', VAPOR DIFFUSION, SITTING DROP, temperature 277K