3QBY
Crystal structure of the PWWP domain of human Hepatoma-derived growth factor 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-03 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97800 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.527, 41.681, 156.749 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.950 |
| R-factor | 0.218 |
| Rwork | 0.216 |
| R-free | 0.25280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eae |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.210 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 | 1.970 |
| High resolution limit [Å] | 1.940 | 5.250 | 1.940 |
| Rmerge | 0.039 | 0.031 | 0.182 |
| Number of reflections | 21026 | ||
| <I/σ(I)> | 23.4 | ||
| Completeness [%] | 99.9 | 99.4 | 98.3 |
| Redundancy | 6.4 | 6.1 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 291 | 2.5 M ammonium sulfate, 0.1 M sodium acetate, peptide ligand was added at 0.005 M concentration, pH 4.6, vapor diffusion, temperature 291K, VAPOR DIFFUSION |
