3QB2
The Crystal Structure of Immunity Factor for SPN (IFS)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-01 |
| Detector | NOIR-1 |
| Wavelength(s) | 0.97901, 0.97932, 0.99504 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 108.032, 108.032, 147.047 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.780 - 2.500 |
| R-factor | 0.26042 |
| Rwork | 0.259 |
| R-free | 0.28986 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.101 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.800 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 34514 |
| Completeness [%] | 100.0 |
| Redundancy | 8.24 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 1.6-2.0 ammonium sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
