3QAY
Catalytic domain of CD27L endolysin targeting Clostridia Difficile
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.507, 69.737, 78.818 |
| Unit cell angles | 101.72, 102.00, 105.24 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.18698 |
| Rwork | 0.184 |
| R-free | 0.24571 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xov |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.983 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.900 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.137 | 0.310 |
| Number of reflections | 50589 | |
| <I/σ(I)> | 8.9 | 2.3 |
| Completeness [%] | 88.3 | 69.8 |
| Redundancy | 1.8 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 292 | 0.1 M Tris-HCl, 0.2 M LiSO4, 20 % PEG 4000, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
