3QAO
The crystal structure of the N-terminal domain of a MerR-like transcriptional regulator from Listeria monocytogenes EGD-e
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 56.769, 56.769, 113.108 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.088 - 1.874 |
| R-factor | 0.1951 |
| Rwork | 0.193 |
| R-free | 0.22790 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.911 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.100 | 1.910 |
| High resolution limit [Å] | 1.874 | 1.880 |
| Rmerge | 0.093 | 0.569 |
| Number of reflections | 17734 | |
| <I/σ(I)> | 42.5 | 3.5 |
| Completeness [%] | 98.7 | 100 |
| Redundancy | 7.1 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.2M Potassium Acetate, 20% (w/v) 3350, 10ug/ml chemtrypsin, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
