3Q9C
Crystal Structure of H159A APAH complexed with N8-acetylspermidine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 |
| Unit cell lengths | 117.461, 120.154, 118.448 |
| Unit cell angles | 98.47, 94.41, 115.89 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.1859 |
| Rwork | 0.186 |
| R-free | 0.22710 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3q9f |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.217 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.370 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.109 | 0.361 |
| Number of reflections | 219596 | |
| <I/σ(I)> | 7.472 | 2.059 |
| Completeness [%] | 86.9 | 96.9 |
| Redundancy | 2.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | 1.0 M sodium phosphate, 0.8 M potassium phosphate, 0.2 M lithium sulfate, 0.1 M CAPS pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
