3Q90

Crystal structure of the NTF2 domain of Ras GTPase-activating protein-binding protein 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-20
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8726
Spacegroup name	P 21 21 21
Unit cell lengths	42.850, 71.410, 88.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.290 - 1.700
R-factor	0.215
Rwork	0.214
R-free	0.23760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1z02
RMSD bond length	0.010
RMSD bond angle	0.980
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.9.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.800
High resolution limit [Å]	1.700	1.700
Number of reflections	30582
<I/σ(I)>	17.17	3.48
Completeness [%]	99.8	99.8
Redundancy	6.17	3.67

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	25% w/v PEG3350, 0.2M AMMONIUM ACETATE, 0.1M BIS-TRIS, VAPOR DIFFUSION, SITTING DROP, temperature 277K, pH 5.5