3Q8D

E. coli RecO complex with SSB C-terminus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-17
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97953
Spacegroup name	I 2 2 2
Unit cell lengths	100.199, 118.569, 145.119
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.300
R-factor	0.19678
Rwork	0.195
R-free	0.23406
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.005
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.064	0.653
Number of reflections	38294
<I/σ(I)>		1.8
Completeness [%]	98.4	87.2
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K