3Q87
Structure of E. cuniculi Mtq2-Trm112 complex responible for the methylation of eRF1 translation termination factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.214 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.115, 74.316, 96.754 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.377 - 1.997 |
| R-factor | 0.2051 |
| Rwork | 0.202 |
| R-free | 0.25580 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.106 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.220 |
| High resolution limit [Å] | 1.997 | 2.000 |
| Number of reflections | 33750 | |
| <I/σ(I)> | 8.3 | 2 |
| Completeness [%] | 97.9 | 96.9 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 310 | 20% PEG4000, 10% isopropanol, 100mM Hepes, pH 7.5, VAPOR DIFFUSION, temperature 310K |
