3Q7T

2.15A resolution structure (I41 Form) of the ChxR receiver domain from Chlamydia trachomatis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-01-01
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.5400
Spacegroup name	I 41
Unit cell lengths	53.720, 53.720, 190.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.670 - 2.150
R-factor	0.2071
Rwork	0.205
R-free	0.24690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.079
Data reduction software	d*TREK
Data scaling software	d*TREK (9.9.3L)
Phasing software	PHASER (2.1.4)
Refinement software	PHENIX (1.6.1_357)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.670	29.650	2.230
High resolution limit [Å]	2.150	4.630	2.150
Rmerge	0.070	0.046	0.429
Total number of observations		5549	5338
Number of reflections	14560
<I/σ(I)>	8.6	22.9	2.5
Completeness [%]	100.0	100	100
Redundancy	3.69	3.71	3.61

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	4 M NaCl, 5 % isopropanol, 0.1 M Na/K Phosphate, pH 7.0, VAPOR DIFFUSION, temperature 293K