3Q7S
2.1A resolution structure of the ChxR receiver domain containing I3C from Chlamydia trachomatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-01-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 149.766, 41.086, 45.069 |
| Unit cell angles | 90.00, 106.10, 90.00 |
Refinement procedure
| Resolution | 28.138 - 2.100 |
| R-factor | 0.199 |
| Rwork | 0.196 |
| R-free | 0.25510 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.698 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.122 | 0.467 |
| Number of reflections | 15613 | |
| <I/σ(I)> | 13.1 | 2.8 |
| Completeness [%] | 98.8 | 96.2 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 20 % PEG 3350, 0.2 M sodium thiocyanate, vapor diffusion, temperature 277K |
