3Q7R
1.6A resolution structure of the ChxR receiver domain from Chlamydia trachomatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-01 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 149.867, 41.268, 45.171 |
| Unit cell angles | 90.00, 105.52, 90.00 |
Refinement procedure
| Resolution | 23.660 - 1.600 |
| R-factor | 0.1894 |
| Rwork | 0.188 |
| R-free | 0.21020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.513 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.660 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.039 | 0.296 |
| Number of reflections | 35320 | |
| <I/σ(I)> | 13.5 | 3.1 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 20% PEG 3350, 0.2M sodium thiocyanate, vapor diffusion, temperature 277K |
