3Q7B

Exonuclease domain of Lassa virus nucleoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.1159
Spacegroup name	P 21 21 21
Unit cell lengths	44.998, 68.233, 79.006
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.000 - 2.000
R-factor	0.1925
Rwork	0.189
R-free	0.25370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.005
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHENIX (1.6_289)
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall
Low resolution limit [Å]	39.503
High resolution limit [Å]	1.700
Number of reflections	16951
Completeness [%]	97.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	0.4M potassium phosphate, 20% PEG 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K