3Q72

Crystal Structure of Rad G-domain-GTP Analog Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	110
Detector technology	CCD
Collection date	2008-11-20
Detector	ADSC QUANTUM 4
Wavelength(s)	0.934
Spacegroup name	P 31
Unit cell lengths	39.084, 39.084, 154.671
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.848 - 1.655
R-factor	0.1762
Rwork	0.172
R-free	0.22530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.092
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX ((AutoMR))
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.680
High resolution limit [Å]	1.650	4.480	1.650
Rmerge	0.037	0.025	0.337
Number of reflections	30044
<I/σ(I)>	31.8	38	2
Completeness [%]	94.6	70.1	59.9
Redundancy	3.2	3.1	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.7	292	25% PEG 400, 5% ethylene glycol 0.1M MES, 0.2M calcium acetate,, pH 5.7, VAPOR DIFFUSION, SITTING DROP, temperature 292K