3Q6S
The crystal structure of the heterochromatin protein 1 beta chromoshadow domain complexed with a peptide from Shugoshin 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9789112 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 76.816, 108.893, 41.938 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.408 - 1.930 |
| R-factor | 0.1922 |
| Rwork | 0.190 |
| R-free | 0.22870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.318 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.800 | 1.960 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Number of reflections | 26940 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.6 | 293 | 0.1 M Bis-tris, 0.2 M NaCl, 21% (w/v) PEG 3350, pH 6.6, VAPOR DIFFUSION, temperature 293K |
