3Q63
X-ray crystal structure of protein MLL2253 from Mesorhizobium loti, Northeast Structural Genomics Consortium Target MlR404.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97890 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.116, 148.122, 87.391 |
| Unit cell angles | 90.00, 98.33, 90.00 |
Refinement procedure
| Resolution | 19.990 - 2.000 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.24400 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX (then SOLVE/RESOLVE) |
| Refinement software | CNS (1.2 & XtalView) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.147 | 0.300 |
| Number of reflections | 136539 | |
| <I/σ(I)> | 14.82 | 3.9 |
| Completeness [%] | 97.8 | 96.1 |
| Redundancy | 6 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 277 | Protein solution: 10 mM Tris (pH 7.5), 100 mM sodium chloride, and 5 mM DTT. Reservoir solution: 100mM BIS-TRIS pH 5.5, 25% PEG 3350, and 200mM Lithium sulfate monohydrate, Microbatch, under oil, temperature 277K |
