3Q5X
Structure of proteasome tether
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-04-12 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 143.440, 36.200, 53.990 |
| Unit cell angles | 90.00, 108.17, 90.00 |
Refinement procedure
| Resolution | 34.990 - 2.980 |
| R-factor | 0.262 |
| Rwork | 0.262 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3KRH |
| RMSD bond length | 0.020 |
| RMSD bond angle | 3.000 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.000 |
| High resolution limit [Å] | 2.980 |
| Rmerge | 0.070 |
| Number of reflections | 5346 |
| <I/σ(I)> | 10 |
| Completeness [%] | 95.6 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 2 M AMMONIUM SULFATE, PH 7.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K |
