3Q5W
Structure of proteasome tether
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9790, 0.9780, 0.939 |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.660, 51.180, 71.330 |
| Unit cell angles | 103.92, 100.02, 98.00 |
Refinement procedure
| Resolution | 67.600 - 2.750 |
| R-factor | 0.231 |
| Rwork | 0.231 |
| R-free | 0.27600 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.000 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 67.600 |
| High resolution limit [Å] | 2.750 |
| Rmerge | 0.065 |
| Number of reflections | 11764 |
| <I/σ(I)> | 19 |
| Completeness [%] | 94.0 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | TASCIMATE, PH 7.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K |
