3Q5O
Crystal structure of human titin domain M10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-02 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.9334 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 118.770, 118.770, 85.220 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.722 - 2.050 |
| R-factor | 0.1903 |
| Rwork | 0.189 |
| R-free | 0.21530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3knb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.003 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.048 | 0.283 |
| Number of reflections | 22628 | |
| <I/σ(I)> | 20.89 | 4.43 |
| Completeness [%] | 99.3 | 97.2 |
| Redundancy | 5.9 | 4.13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 277 | 0.1M bistris p 6.8, 55% MPD, 0.2M magnesium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
