3Q5F
Crystal structure of the Salmonella transcriptional regulator SlyA in complex with DNA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Detector technology | CCD |
| Collection date | 2010-03-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.20610, 1.25494, 1.25525 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 59.566, 59.566, 291.177 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.099 - 2.960 |
| R-factor | 0.2189 |
| Rwork | 0.213 |
| R-free | 0.26620 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.354 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.080 |
| High resolution limit [Å] | 2.960 | 6.390 | 2.960 |
| Rmerge | 0.081 | 0.036 | 0.260 |
| Number of reflections | 11205 | ||
| <I/σ(I)> | 10.4 | ||
| Completeness [%] | 94.4 | 99.1 | 63.2 |
| Redundancy | 12.5 | 12.2 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.74 | 298 | 20% PEG 4000, 0.1M sodium chloride, 0.05M magnesium chloride, 0.1M cacodylate, 2% benzamidine hydrochloride, pH 6.74, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 10 | 1 | 2 | benzamidine hydrochloride | ||
| 2 | 1 | 1 | sodium chloride | ||
| 3 | 1 | 1 | magnesium chloride | ||
| 4 | 1 | 1 | cacodylate | ||
| 5 | 1 | 1 | benzamidine hydrochloride | ||
| 6 | 1 | 2 | PEG 4000 | ||
| 7 | 1 | 2 | sodium chloride | ||
| 8 | 1 | 2 | magnesium chloride | ||
| 9 | 1 | 2 | cacodylate |
