3Q4O
Crystal Structure of a deletion mutant(11-185) of hypothetical protein MJ0754 determined to 1.34A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 51.915, 79.122, 93.869 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.710 - 1.340 |
| R-factor | 0.1891 |
| Rwork | 0.187 |
| R-free | 0.22760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q4n |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.436 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.390 |
| High resolution limit [Å] | 1.340 | 2.890 | 1.340 |
| Number of reflections | 40164 | ||
| Completeness [%] | 91.5 | 94.8 | 52.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.1M Bis-tris propane, 22% PEG3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
