3Q4L
Structure of a small peptide ligand bound to E.coli DNA sliding clamp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Collection date | 2007-10-01 |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.840, 79.570, 81.640 |
| Unit cell angles | 65.28, 75.26, 82.22 |
Refinement procedure
| Resolution | 39.000 - 1.950 |
| R-factor | 0.2005 |
| Rwork | 0.199 |
| R-free | 0.23210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | BUSTER-TNT (BUSTER 2.8.0) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 2.000 | ||
| High resolution limit [Å] | 1.950 | 10.000 | 1.950 |
| Rmerge | 0.061 | 0.024 | 0.724 |
| Number of reflections | 54138 | 328 | 3818 |
| <I/σ(I)> | 12.99 | 33.5 | 1.9 |
| Completeness [%] | 96.3 | 78.3 | 93.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 298 | 0.1 M MES PH 6.0, 0.1M CaCl2, 30% PEG 400, vapor diffusion, temperature 298K |
