3Q4A
Crystal structure of the TPR domain of CHIP complexed with phosphorylated Smad1 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97924 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 46.132, 77.444, 36.456 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.984 - 1.542 |
| R-factor | 0.1703 |
| Rwork | 0.168 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c2l |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.028 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.000 | 1.570 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.053 | 0.171 |
| Number of reflections | 19797 | |
| <I/σ(I)> | 57.7 | 11.8 |
| Completeness [%] | 99.4 | 97.9 |
| Redundancy | 7.7 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 100mM Bis-tris propane pH 7.0, 40% PEG MME 2000, 1% PEG MME 550, 50mM magnesium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
