3Q48

Crystal structure of Pseudomonas aeruginosa CupB2 chaperone

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-19
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9798
Spacegroup name	C 1 2 1
Unit cell lengths	93.520, 65.780, 87.510
Unit cell angles	90.00, 105.95, 90.00

Refinement procedure

Resolution	28.090 - 2.500
R-factor	0.2011
Rwork	0.198
R-free	0.25910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2co6 1n0l
RMSD bond length	0.019
RMSD bond angle	1.757
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.100	2.640
High resolution limit [Å]	2.500	2.500
Number of reflections	17834
Completeness [%]	98.3	95.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	298	14%(w/v) PEG4000, 10%(v/v) isopropanol, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K