3Q2W

Crystal structure of mouse N-cadherin ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4C
Synchrotron site	NSLS
Beamline	X4C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-31
Detector	MAR CCD 165 mm
Wavelength(s)	0.9793
Spacegroup name	C 2 2 21
Unit cell lengths	91.372, 111.648, 262.107
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.947 - 3.200
R-factor	0.2315
Rwork	0.230
R-free	0.26720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.176
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	3.200
Number of reflections	21590
Completeness [%]	88.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	25% PEG8000, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K