3Q2T

Crystal Structure of CFIm68 RRM/CFIm25/RNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-03-23
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 21 3
Unit cell lengths	138.406, 138.406, 138.406
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.977 - 3.061
R-factor	0.2137
Rwork	0.211
R-free	0.28580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bho
RMSD bond length	0.006
RMSD bond angle	0.966
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.6_289)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	3.170
High resolution limit [Å]	3.060	6.520	3.060
Rmerge	0.146	0.053	1.000
Number of reflections	16919
<I/σ(I)>	8
Completeness [%]	100.0	100	100
Redundancy	36.8	44.4	27.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	20% PEG 3350, 0.2M Magnesium Formate, 0.05M HEPES pH 7.0, vapor diffusion, hanging drop, temperature 298K