3Q2R
crystal structure of sGLIPR1 soaked with zinc chloride
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-08 |
| Detector | OXFORD ONYX CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 85.920, 79.720, 38.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.788 - 2.200 |
| R-factor | 0.1601 |
| Rwork | 0.158 |
| R-free | 0.19200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rc9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.982 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_777) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.790 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.179 | 0.621 |
| Number of reflections | 18698 | |
| <I/σ(I)> | 7.9 | 2.5 |
| Completeness [%] | 99.4 | 99.1 |
| Redundancy | 5.8 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 293 | 0.085M sodium cacodylate, 25.5%w/v PEG8000, 0.17M Ammonium sulfate, 15% glycerol 0.2mM Zinc Chloride, pH 6.5, VAPOR DIFFUSION, temperature 293K |
