3Q2B

Crystal Structure of an Actin Depolymerizing Factor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Detector technology	CCD
Wavelength(s)	0.95369
Spacegroup name	P 43 21 2
Unit cell lengths	38.970, 38.970, 142.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.556 - 1.600
R-factor	0.1942
Rwork	0.193
R-free	0.21820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f7s
RMSD bond length	0.009
RMSD bond angle	1.203
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.6.3_473))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.600	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.067	0.999
Number of reflections	15439
<I/σ(I)>	3.2	3.2
Completeness [%]	80.0	80
Redundancy	13.6	13.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	295	1.8M (NH4)2SO4, 0.2M KNa tartrate, 0.1M NaOAc, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K