3Q1N
Crystal structure of a galactose mutarotase-like protein (LSEI_2598) from Lactobacillus casei ATCC 334 at 1.61 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-24 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91837,0.97903,0.97881 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.152, 67.076, 93.978 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.530 - 1.610 |
R-factor | 0.157 |
Rwork | 0.155 |
R-free | 0.18200 |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.549 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.529 | 1.670 |
High resolution limit [Å] | 1.610 | 1.610 |
Rmerge | 0.076 | 0.630 |
Number of reflections | 40969 | |
<I/σ(I)> | 11.86 | 2.2 |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 3.582 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.3 | 277 | 0.20M NH4NO3, 20.00% PEG-3350, No Buffer pH 6.3, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |