3Q1J

Crystal structure of tudor domain 1 of human PHD finger protein 20

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Detector technology	IMAGE PLATE
Collection date	2010-10-06
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	60.498, 41.062, 28.632
Unit cell angles	90.00, 110.33, 90.00

Refinement procedure

Resolution	19.080 - 2.350
R-factor	0.2074
Rwork	0.205
R-free	0.26010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdb entries 2rhu 2eqm
RMSD bond length	0.010
RMSD bond angle	1.040
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	BUSTER-TNT (BUSTER 2.8.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	2.430
High resolution limit [Å]	2.350	5.040	2.350
Rmerge	0.100	0.059	0.654
Number of reflections	2828
<I/σ(I)>	9.1
Completeness [%]	99.8	99.3	100
Redundancy	3.4	3.3	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	25% PEG-3350, 0.2M ammonium acetate, 0.1M bis-tris. Prior to freezing, a cluster of thin rods was transferred to a drop of paratone oil. A fragment of the cluster was frozen in a stream of cold nitrogen., pH 5.5, vapor diffusion, sitting drop, temperature 291K, VAPOR DIFFUSION, SITTING DROP