3Q1D
The B-box domain of Trim54
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97951 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 47.276, 47.276, 87.047 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.823 - 2.150 |
| R-factor | 0.18518 |
| Rwork | 0.182 |
| R-free | 0.25343 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ddt |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.417 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 3560 | |
| <I/σ(I)> | 32.3 | 5.3 |
| Completeness [%] | 99.9 | 98.3 |
| Redundancy | 23.6 | 15.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 1.4M SODIUM CITRATE, 0.1M HEPES PH 7.5, 5% MPD, 0.57 MICROMOLAR TRYPSIN, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
