3Q13
The Structure of the Ca2+-binding, Glycosylated F-spondin Domain of F-spondin, A C2-domain Variant from Extracellular Matrix
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 120.244, 120.244, 86.362 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 104.130 - 1.950 |
| R-factor | 0.16885 |
| Rwork | 0.167 |
| R-free | 0.20982 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB:3D34 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.551 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 104.130 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.126 | 0.646 |
| Number of reflections | 27484 | |
| <I/σ(I)> | 23.4 | 3.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.1 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2M Lithium sulfate monohydrate, 25% (w/v) PEG 3350, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
