3Q0N
Crystal structure of the PUMILIO-homology domain from Human PUMILIO1 in complex with erk2 NRE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-05 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 261.672, 37.677, 82.551 |
| Unit cell angles | 90.00, 102.68, 90.00 |
Refinement procedure
| Resolution | 22.886 - 2.400 |
| R-factor | 0.2052 |
| Rwork | 0.202 |
| R-free | 0.25950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.864 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.4_6)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.900 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 31306 | |
| Completeness [%] | 99.2 | 94.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 293 | 15-20% (w/v) PEG 3350, 100 mM Li2SO4, and 100 mM Na3Citrate pH 5.5-6.0 , VAPOR DIFFUSION, temperature 293K |
