3Q0M

Crystal structure of the PUMILIO-homology domain from Human PUMILIO1 in complex with p38alpha NREb

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2009-03-25
Detector	RIGAKU SATURN 92
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	35.857, 59.654, 333.584
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.800 - 2.705
R-factor	0.216
Rwork	0.212
R-free	0.29820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.236
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.4_6))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.800	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	16244
Completeness [%]	78.7	80.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	293	15-20% (w/v) PEG 3350, 100 mM Li2SO4, and 100 mM Na3Citrate pH 5.5-6.0, VAPOR DIFFUSION, temperature 293K