3Q0B

Crystal structure of SUVH5 SRA- fully methylated CG DNA complex in space group P42212

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918,0.97929,0.9900
Spacegroup name	P 42 21 2
Unit cell lengths	76.566, 76.566, 73.904
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.587 - 2.200
R-factor	0.2184
Rwork	0.217
R-free	0.25180
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.198
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.047	0.510
Number of reflections	11770
<I/σ(I)>	49.4	4.2
Completeness [%]	99.7	99.9
Redundancy	11.4	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	19% PEG 4000, 100 mM Tris-HCl, pH 7.5 and 40 mM CaCl2, VAPOR DIFFUSION, SITTING DROP, temperature 291K
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	19% PEG 4000, 100 mM Tris-HCl, pH 7.5 and 40 mM CaCl2, VAPOR DIFFUSION, SITTING DROP, temperature 291K
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	19% PEG 4000, 100 mM Tris-HCl, pH 7.5 and 40 mM CaCl2, VAPOR DIFFUSION, SITTING DROP, temperature 291K